DNA sequence and expression of the B95-8 Epstein—Barr virus genome | Semantic Scholar (2024)

Protein sequence comparison has revealed an analogue of the HSV glycoprotein B in the EBV genome and weak transcription enhancers have been detected in EBV and more mRNAs have been mapped.

Site-directed mutagenesis of this DNA target suggests that BZLF1 may work partly by overcoming a cellular repressor of viral transcription.

The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B 95-8 DNA to make a recombinant vaccinia virus that was shown to express a relatively high level of LMP in an authentic fashion.

...

...

The sequence of the 17,166-base-pair EcoRI C fragment of Epstein-Barr virus DNA, cell line B95-8, was determined and upstream sequences near the three promoters show striking hom*ologies that may be involved in transcriptional control.

Four RNA polymerase II promoters have been mapped in the DNA sequence of the EcoRI‐H and ‐Dhet fragments of B95‐8 Epstein‐Barr virus and deletion mapping suggests that DNA sequence hom*ologies between some of the promoters lie in the same region as essential upstream promoter elements.

The primary structure of two of the repeat sequences, the 3072 bp large internal repeat, or BamHI-W repeat, and a smaller 125 bp, G, C-rich NotI repeat, are presented and their structures and possible functions are discussed.

Two of the Sal I fragments and all of the internal BamHI fragments (with the exception of BamHI c, a 0.6 x 10(6) dalton fragment) of Epstein-Barr virus (EBV) DNA have been cloned in pBR322 to derive a complete map of the Bam HI fragments of EBV DNA and to align the BamHI, EcoRI, HindIII, and SalI cleavage sites in EBVDNA.

Northern blot analysis indicates that one of the late promoters initiates two transcripts whose size differences are due to different splicing patterns, which code for the 350/300 and 250/200 kd envelope glycoproteins of EBV.

Over 30 viral-specified polypeptides are translated in vitro from RNA of cells productively infected with Epstein-Barr virus, and several are likely to be major structural components of the virus.

The results confirmed that the most abundant cytoplasmic RNA species in such cells is specified by a small region of the genome defined by the EcoRI J fragment.

The location of each cloned DNA fragment in the viral genome has been determined, providing a more detailed physical map of the genome than has been available previously.

...

...